zhaosf-RGF1通过ROS信号控制根分生组织的大小

时间:2019-12-06 13:40 来源: www.seosem.ws

本期文章:《自然》:Online/在线发表

近日,美国杜克大学教授Philip N. Benfey及其研究团队发现根分生组织生长因子1(RGF1)通过ROS信号控制根分生组织的大小。该项研究成果2019年12月4日在线发表于《自然》。

研究人员报告了一系列遵循RGF1活动的信号事件。研究人员发现,RGF1受体通路控制了拟南芥根的发育区域中活性氧(ROS)的分布。研究人员确定了以前未表征的转录因子,RGF1诱导转录因子1(RITF1),在介导RGF1信号传导中起着核心作用。操纵RITF1表达导致ROS沿着根发育区重新分布。ROS分布的变化反过来增强了PLETHORA2蛋白(根干细胞的主要调节者)的稳定性。因此,这些结果清楚地描述了由RGF1引发的信号传导级联,将该多肽与调节ROS的机制联系起来。

据介绍,植物的干细胞微环境和根分生组织的大小通过细胞间相互作用和涉及肽激素、RGF1的信号网络来维持。了解RGF1如何调节根分生组织的发育对于了解干细胞功能至关重要。尽管已鉴定出RGF1的五个受体,但下游信号传导机制仍然未知。

附:英文原文

Title: RGF1 controls root meristem size through ROS signalling

Author: Masashi Yamada, Xinwei Han, Philip N. Benfey

Issue&Volume: 2019-12-04

Abstract: The stem cell niche and the size of the root meristem in plants are maintained by intercellular interactions and signalling networks involving a peptide hormone, root meristem growth factor 1 (RGF1)1. Understanding how RGF1 regulates the development of the root meristem is essential for understanding stem cell function. Although five receptors for RGF1 have been identified24, the downstream signalling mechanism remains unknown. Here we report a series of signalling events that follow RGF1 activity. We find that the RGF1-receptor pathway controls the distribution of reactive oxygen species (ROS) along the developmental zones of the Arabidopsis root. We identify a previously uncharacterized transcription factor, RGF1-INDUCIBLE TRANSCRIPTION FACTOR 1 (RITF1), that has a central role in mediating RGF1 signalling. Manipulating RITF1 expression leads to the redistribution of ROS along the root developmental zones. Changes in ROS distribution in turn enhance the stability of the PLETHORA2 protein, a master regulator of root stem cells. Our results thus clearly depict a signalling cascade that is initiated by RGF1, linking this peptide to mechanisms that regulate ROS. RITF1, a newly identified plant transcription factor, links signalling through the peptide hormone RGF1 to the balance of reactive oxygen species and thereby enhances the stability of another transcription factor, PLETHORA2, a master regulator of root stem cells.

DOI: 10.1038/s41586-019-1819-6

Source:https://www.nature.com/articles/s41586-019-1819-6

期刊信息

Nature:《自然》,创刊于1869年。隶属于施普林格·自然出版集团,最新IF:43.07
官方网址:
投稿链接: